Dr. Fu-Chyun “Clay” Chu
ISI Vice President
I am the vice president of Invertebrate Studies Institute and working with insect for more than 10 years. I have a PhD in Entomology and Plant Pathology at North Carolina State University (NCSU) on 2017. My expertise are insect molecular and genetic researches including DNA/RNA sequencing and biotechnology applications in insect. I have more than 15 years of experience on insect rearing since I was an undergraduate student in Taiwan. During my PhD study, I am also volunteer in the entomology outreach program in NCSU to educate the young generation knowing how special and interest insects are. This genome project is a combination of my work and interesting. It can not only provide more information about this unique species, but also creating more potential opportunities on medical and chemical researches.
Curriculum Vitae
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Highlights · 4 publications, including 3 first-author · 13 years of experience with molecular biology and genetic engineering · Southern SARE Graduate Student Grant Award, 2014 · 5 invited talks in international conferences · 20 scientific presentations including national and international conferences
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Education |
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North Carolina State University, Raleigh, North Carolina PhD in Department of Entomology and Plant Pathology Minor: Biotechnology program Thesis: Developing Transposon Helper Strains for Two Agriculture Pests; Western Corn Rootworm and Spotted Wing Drosophila Major Advisor: Dr. Marcé Lorenzen & Dr. Fred Gould Committee Members: Dr. Max Scott & Jim Mahaffey |
Graduated: May2017 |
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National Ilan University, Ilan, Taiwan Bachelors of Science in Animal Science Senior Thesis: Effect of polymorphism of prolactin receptor (PRLR) and Porcine stress syndrome (PSS) on in vitro maturation and fertilization of pig oocytes and in vitro culture of pig zygotes. Thesis Advisor: Dr. Ming-Cheng Chen |
Graduated: July 2007 |
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Grants & Awards |
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Southern SARE Graduate Student Grant Award |
2014 |
$11,000 |
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Experiences |
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All Things Bugs LLC, Oklahoma City, Oklahoma Senior Scientist Developing, engineering, breeding, and evaluating the nutrient enhanced transgenic mealworm and house cricket strains. · Set up a new molecular lab and starter insect colonies with special requirement for the company · Design the transgenesis work including: DNA construct design, microinjection and breeding protocols · Performed RNA-Seq, genomic sequencing, bioinformatics for both insect species. · Published paper and presented research result in conferences |
Jul 2018-present |
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North Carolina State University, Raleigh, North Carolina Post-doc and Graduate Research Assistant Engineering, breeding, and evaluating multiple transgenic insect species and developing genetic pest control strategies. · Published paper and presented research result in conferences · Developed transgenic insect strains using transposable elements and CRISPR in five species. · Performed RNA-Seq, genomic sequencing, genome editing, and RNA interference for functional genomics. |
Aug 2011-Sep 2018 |
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National Taiwan University, Taipei, Taiwan Research Assistant Examined the feeding behaviors of predatory mite, Phytoseiulus persimilis; and larvae competition of two pea weevil species.
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Oct 2009-Aug 2010 |
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National Ilan University, Ilan, Taiwan Undergraduate Student Worker Provided critical support to senior laboratory personal by conducting cell culturing, tissue fixation, and sample staining.
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May 2006-Jul 2007 |
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Publications |
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Oppert, B., Chu, F.C., Reyna, S., Pinzi, S., Adrianos, S., Perkin, L. and Lorenzen, M., 2019. Effects of targeting eye color in Tenebrio molitor through RNA interference of tryptophan 2, 3‐dioxygenase (vermilion): Implications for insect farming. Archives of insect biochemistry and physiology, 101(1), p.e21546. |
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Chu, F.C., Klobasa, W., Wu, P., Pinzi, S., Grubbs, N., Gorski, S., Cardoza, Y. and Lorenzen, M.D., 2017. Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera. Insect molecular biology, 26(4), pp.440-452. |
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Chu, F.C., Klobasa, W., Grubbs, N. and Lorenzen, M.D., 2018. Development and use of a piggyBac‐based jumpstarter system in Drosophila suzukii. Archives of insect biochemistry and physiology, 97(3), p.e21439 |
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Chu, F.C., Wu, P.S., Pinzi, S., Grubbs, N. and Lorenzen, M.D., 2018. Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing. JoVE (Journal of Visualized Experiments), (134), p.e57497. |
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Manuscripts In Preparation or Review |
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Dossey, A. T., Chu, F.C., and Opper, B., (In Prep). Sustainable Nutrition and a Tale of 2 Cricket Genome |
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Chu, F.C., Wu, P.S., Pinzi, S., Grubbs, N., Cohen, A. C., and Lorenzen, M.D., (In Prep). An optimized small-scale rearing system to support embryonic microinjection protocol for western corn rootworm, Diabrotica virgifera virgifera |
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Grubbs, N., Chu, F.C., and Lorenzen, M.D., (In Prep). Window to the Fluorescence: The White Eye-Color Gene of Western Corn Rootworm, Diabrotica virgifera virgifera |
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Areas of Expertise |
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Molecular Biology Genetic Engineering CRISPR/Cas9 Genome Editing Software: Microsoft Office, BlastStation, Vector NTI |
DNA construction DNA/RNA Sequencing RNA interference PCR, RT-PCR, digital-droplet PCR |
Microinjection of Embryos Insect rearing Insect dissection Phenotype screening |
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Current Research Interests |
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· Genetic engineering in insects using CRISPR/Cas9 system to change phenotype, behavior, or nutrient content. |
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· Insect genome/RNA sequencing and functional genomics. |
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Broader Impacts and Academic Volunteering |
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Invertebrate Studies Institute, Oklahoma City, Oklahoma Vice President · Manage the insect zoo for the company · Helping company developing and decision making · Grant proposal writing |
Feb 2019-present |
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Entomology Outreach Program, Raleigh, North Carolina Bug Keeper · Rearing exotic insects for outreach program · Select and bring in new insect species to program |
Aug 2016-Jul 2017 |
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Bug Fest, Raleigh, North Carolina Volunteer in NCSU Entomology booth · Showing alive and dead insects. · Showing dead transgenic insects and explain the technology |
2017 and 2012 |
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Student voice in NABC, Cornell University, Ithaca, New York Student voice attender · Discuss the impact of current biotechnology to world · Communicate with professors with how young generation think of transgenic technologies. |
2014 |
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Invited Presentations |
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Developing Insects for Food, Feed and Bioproduction: White-Eye Marker for CRISPR/Cas9 in Crickets and Mealworms |
F. Chu |
Plant & Animal Genome Conference XXVIII, San Diego, CA |
2020 |
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The ABCs of Using CRISPR in Non-Model Organisms |
F. Chu |
Plant & Animal Genome Conference XXVIII, San Diego, CA |
2020 |
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CRISPR/Cas9 in the mealworm, Tenebrio molitor, toward improvement as a crop |
F. Chu |
Plant & Animal Genome Conference XXVII, San Diego, CA |
2019 |
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Emerging technologies for genetic manipulation of western corn rootworm |
F. Chu |
26th IWGO Conference, Beijing, China |
2017 |
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New Western corn rootworm strains for use in genome-wide mutagenesis |
F. Chu, M.D. Lorenzen |
Corn Academic Summit. St. Charles. MO |
2016 |
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Development of transgenic helper/donor Diabrotica virgifera virgifera strains for use in genome-wide mutagenesis |
F. Chu, M.D. Lorenzen |
Corn Academic Summit. St. Louis. MO |
2015 |
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Targeted piggyBac insertion in a pest genome |
F. Chu |
Biolunch. Raleigh, NC |
2015 |
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Genetics and genomics of Tribolium Medea elements |
F. Chu, M.D. Lorenzen |
11th International Working Conference on Stored Product Protection. Chiang Mai, Thailand |
2014 |
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Development of a germline transformation system for the western corn rootworm, Diabrotica virgifera virgifera |
F. Chu, S. Gorski, Y. J. Cardoza M.D. Lorenzen |
Corn Academic Summit, St. Louis. MO |
2014 |
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Development of RNAi-based Dominant Markers for use in Genetic Pest Management |
F. Chu, M.D. Lorenzen |
Entomology Graduate Student Symposium, Raleigh, NC |
2012 |
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Scientific 3rd Friday, Raleigh, NC |
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Poster Presentations |
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Reference Quality Insect Genomes: Applications for Sustainable Protein and Biodiversity |
A. T. Dossey, F. Chu, B. Oppert |
Annual G10K-VGP/EBP 2019, New York city, NY |
2019 |
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Transgenic strains for genome-wide mutagenesis of two serious agricultural pests, Drosophila suzukii and Diabrotica virgifera virgifera |
F. Chu, W. A. Klobasa, M.D. Lorenzen |
Research Symposium at Bayer CropScience, Raleigh, NC. |
2016 |
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The best of two worlds: the use of CRISPR/Cas9 and transposable elements to generate a genome-wide mutagenesis system for the Western corn rootworm, Diabrotica virgifera virgifera |
F. Chu, S. Gorski, Y. J. Cardoza M.D. Lorenzen |
Ninth Annual Arthropod Genomics Symposium. Manhattan, KS |
2015 |
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Insect Genetic Technology Research Coordination Network. Manhattan, KS. |
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Genetic Engineering Research at NC State. Raleigh, NC. |
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Development of a germline transformation system for the western corn rootworm, Diabrotica virgifera virgifera |
F. Chu, S. Gorski, Y. J. Cardoza M.D. Lorenzen |
National Agricultural Biotechnology Council, Ithaca, NY |
2014 |
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2014 Graduate Student Research Symposium, Raleigh, NC |
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Plant and Animal Genome XXIIConference, San Diego, CA |
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Development of RNAi-based Dominant Markers for use in Genetic Pest Management |
F. Chu, M.D. Lorenzen |
6th Annual Arthropod Genomics Symposium, Kansas City, MO |
2012 |
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References: |
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Dr. Marce Lorenzen 785-313-0712 mdlorenz@ncsu.edu Associate Professor North Carolina State University, Raleigh, NC, 27695 PhD major advisor
Dr. Allen Cohen 919-513-0567 accohen@ncsu.edu Research Professor North Carolina State University, Raleigh, NC, 27695 A close professor in the Entomology department in NCSU
Dr. Fred Gould 919-515-1647 fred_gould@ncsu.edu William Neal Reynolds Professor of Agriculture PhD major advisor North Carolina State University, Raleigh, NC, 27695
Dr. Brenda Oppert 785-776-2780 brenda.oppert@usda.gov Research Molecular Biologist USDA-ARS Manhattan, KS, 66502 Research collaborator
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